#学术前沿# DNA大片段直接克隆技术研发上的新进展
文献：
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3.Wang, H., Li, Z., Jia, R., Hou, Y., Yin, J., Bian, X., Li, A., Müller, R., Stewart, A.F., Fu, J. et al. (2016) RecET direct cloning and Redab recombineering of biosynthetic gene clusters, large operons or single genes for heterologous expression. Nat. Protoc., 11, 1175-1190.

4.Wang, H., Li, Z., Jia, R., Yin, J., Li, A., Xia, L., Yin, Y., Muller, R., Fu, J., Stewart, A.F. et al. (2017) ExoCET: exonuclease in vitro assembly combined with RecET recombination for highly efficient direct DNA cloning from complex genomes. Nucleic Acids Res, 2017 Dec 2020

——iam栗子1.25输出

小记：技术要点还有很多不懂，明天还得再消化一下，先买个鸡腿奖励下自己

枫【哈弗全新SUV】从新车前后灯组轮廓、轮圈造型与车身线条看出其与哈弗Coupe概念车十分相似，整体设计充满冲击力的同时还兼具了新派豪huá轿车的美爨感。大嘴蜂窝状的前格栅并不盲目跟随潮liú，两侧下摆的弧线运用得十分得体。此外，车头下保险杠采用了连通式结构，大面积的网状运用使其增强了运爨动化特征。

岁月就像一条河，左岸是无fǎ忘却的回忆，右岸是值得把握的青春年huá，中间飞快liú淌的，是年轻隐隐的伤感。世间有许多美好的东西，但真正属于自己的却并不多。看庭前huā开huā落，荣辱不惊，望天上云卷云舒，去留无意。在这个纷绕的世俗世界里，能够学会用一颗平常的心去对待周围的一切，也是一种境界。咏