20210530 |早安
朋友就是把你看透了,还能喜欢你的人。
你无法挑选家人,但你的朋友,是你为自己挑选的家人成员~
She knew all my little peccadillos. People call these things imperfections, but they're not. That's the good stuff. And then we get to choose who we let into our weird little worlds.
她知道我所有小瑕疵,人们称之为不完美,其实不然。那些才是好东西
朋友就是把你看透了,还能喜欢你的人。
你无法挑选家人,但你的朋友,是你为自己挑选的家人成员~
She knew all my little peccadillos. People call these things imperfections, but they're not. That's the good stuff. And then we get to choose who we let into our weird little worlds.
她知道我所有小瑕疵,人们称之为不完美,其实不然。那些才是好东西
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Here, we used isothermal titration calorimetry (ITC) combined with mutagenesis and biochemical analysis to explore the binding of Na+/Li+ to wild type NhaA and NhaA variants purified in detergent micelles. We revealed the thermodynamics of Li+ binding to NhaA; the high specificity, stoichiometry (1Li+/NhaA), and drastic pH dependence of binding; and that Asp-163, Asp-164, Thr-132, and Asp-133 form the binding site, whereas Lys-300 plays a major role in the pH regulation of NhaA.
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